Expression of prokaryotic and eukaryotic cytochromes c in Escherichia coli

Yeast HIS3 expression in Escherichia coli depends upon fortuitous homology between eukaryotic and prokaryotic promoter elements.

The yeast imidazoleglycerolphosphate dehydratase gene HIS3, when introduced into Escherichia coli, is transcribed and translated with sufficient fidelity to produce functional enzyme. The following lines of evidence indicate that E. coli RNA polymerase recognizes a particular region of HIS3 DNA as a promoter sequence. First, this promoter contains nucleotide sequences that resemble the canonica...

Genetic analysis of prokaryotic and eukaryotic DNA-binding proteins in Escherichia coli.

This report describes an Escherichia coli genetic system that permits bacterial genetic methods to be applied to the study of essentially any prokaryotic or eukaryotic site-specific DNA binding protein. It consists of two parts. The first part is a set of tools that facilitate construction of customized E.coli strains bearing single copy lacZYA reporters that are repressed by a specific target ...

Advances and challenges in recombinant protein expression in Escherichia coli

Multiple low spin forms of the cytochrome c ferrihemochrome. EPR spectra of various eukaryotic and prokaryotic cytochromes c.

1. Despite the same methionine-sulfur:heme-iron:imidazole-nitrogen hemochrome structure observed by x-ray crystallography in four of the seven c-type eukaryotic and prokaryotic cytochromes examined, and the occurrence of the characteristic 695 nm absorption band correlated with the presence of a methionine-sulfur:heme-iron axial ligand in all seven proteins, they fall into two distinct classes...

High-level expression of tetanus toxin fragment C in Escherichia coli

Fragment C is the C-terminal domain of the heavy chain of tetanus toxin that can promote the immune response against the lethal dose of this toxin. Therefore, this portion can be considered as a candidate vaccine against tetanus infection, which occurs by Clostridium tetani. The present study aimed to compare the expression of tetanus toxin fragment C in Escherichia coli  BL21 (DE3) pLysS cells...